Salah Abdel-Ghany

Salah Abdel-GhanySpecial Assistant Professor
Office: E300 Anatomy-Zoology Building
Phone: 970-286-9426 (Cell) 970-491-2189 (Lab)
Education: Ph.D., Colorado State University
Email: salah.abdel-ghany@colostate.edu

In nature, plants are continuously subjected to environmental stresses such as drought, heat, and salinity both individually or in combination. To cope with these stresses, plants have evolved array of molecular reprograming events to sense these changes rapidly and adapt accordingly. Understanding these reprograming events would be highly useful and could be exploited to help engineering plants that are capable of growing well under adverse environmental conditions. These reprograming events include chromatin re-modeling, pre-mRNA splicing and small non-coding microRNAs. We use high-throughput next-generation sequencing (NGS) technologies (RNA-seq, ChIP-seq, DNAseI-seq) in combination with, molecular, biochemical and genetic techniques to accomplish our research goals. Analysis of NGS data is done in collaboration with Asa Ben-Hur’s team in the Department of computer science at Colorado State University.    

Selected Publications

Salah E. Abdel-Ghany, Irene Day, Adam L. Heuberger, Corey D. Broeckling, Anireddy S.N. Reddy (2016): Production of Phloroglucinol, a Platform Chemical, in Arabidopsis using a Bacterial Gene. Scientific reports. 2016; 6: 38483.

Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur, Anireddy S.N. Reddy (2016): A survey of the Sorghum transcriptome using single-molecule long reads. Nature Communication, 7, 2016.Doi:10.1038/ncomms11706.

Brian Fisher, Dmitry Yarmolinsky, Salah Abdel-Ghany, Marinus Pilon, Elizabeth A. Pilon-Smits, Moshe Sagi, Doug Van Hoewyk (2016): Superoxide generated from the glutathione-mediated reduction of selenite damages the iron-sulfur cluster of chloroplastic ferredoxin. Plant Physiology and Biochemistry 106, 228-235.

Ashraf S. A. El-Sayed, Laura E. Ruff, Salah E. Abdel-Ghany, Gul Shad Ali, Sadik Esener (2016): Molecular and Spectroscopic Characterization of Aspergillus flavipes and Pseudomonas putida L-Methionine γ-Lyase in vitro. Applied Biochem Biotechnol. DOI 10. 1007/s 12010-016-2299-x

Salah E. Abdel-Ghany, Maxim Golovkin, and A.S.N. Reddy (2015): Engineering of Plants for the Production of Commercially Important Products: Approaches and Accomplishments. Chapter 28, In Plant Genomics and Biotechnology, Volume II, ISBN 978-81-322-2282-8

Salah E. Abdel-Ghany, Day I, Heuberger AL, Broeckling CD, Reddy AS (2013): Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes. Metabolic Engineering. 2013 Nov; 20:109-20.

Julie Thomas, Saiprasad G. Palusa, Kasavajhala V.S.K. Prasad, Gul Shad Ali, Giridara-Kumar Surabhi, Asa Ben-Hur, Salah E. Abdel-Ghany and Anireddy S.N. Reddy (2012): Identification of an intronic splicing regulatory element involved in auto-regulation of alternative splicing of SCL33 pre-mRNA. The Plant Journal 72, 935–946

Hou-Sung Jung, Yuki Okegawa, Patrick M. Shih, Elizabeth Kellogg, Salah E. Abdel-Ghany, Marinus Pilon, Kimmen Sjölander, Toshiharu Shikanai, and Krishna K. Niyogi (2010): Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport. PLoS One. 5 (7). doi: 1371/journal.pone.0011688.

Salah E. Abdel-Ghany  (2009): Contribution of plastocyanin isoforms to photosynthesis and copper homeostasis in Arabidopsis thaliana grown at different copper regimes. Planta. Vol. 229, Issue (4), 767-779.

Christopher M. Cohu, Salah E. Abdel-Ghany, Kathryn A. Gogolin Reynolds, Alexander M. Onofrio, Jared R. Bodecker, Jeffrey A. Kimbrel, Krishna K. Niyogi and Marinus Pilon (2009): Copper Delivery by the Copper Chaperone for Chloroplast and Cytosolic Copper/Zinc-Superoxide Dismutases: Regulation and Unexpected Phenotypes in an Arabidopsis Mutant. Molecular Plant. Pages 1–15.

Marinus Pilon, Christopher M Cohu, Karl Ravet, Salah E. Abdel-Ghany and Frederic Gaymard (2009): Essential transition metal homeostasis in plants. Current Opinion in Plant Biology, 12:347–357.

Jason L. Burkhead, Kathryn A. Gogolin Reynolds, Salah E. Abdel-Ghany, Christopher M. Cohu and Marinus Pilon (2009): Copper homeostasis. New Phytologist. 182: 799–816

Salah E. Abdel-Ghany  and Pion M. (2008): MicroRNAs-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis. Journal of Biological Chemistry. Vol. 283, Issue 23, 15932-15945.

Hiroaki Yamasaki*, Salah E. Abdel-Ghany*, Christopher M. Cohu, Yoshichika Kobayashi, Toshiharu Shikanai and Marinus Pilon. (2007): Regulation of Copper Homeostasis by micro-RNA.  Journal of Biological Chemistry. Vol. 282, Issue 22, 16369-16378. * Shared First author in the manuscript

Van Hoewyk D, Salah E. Abdel-Ghany, Cohu C, Herbert S, Kugrens P, Pilon M, Pilon-Smits EAH (2007): Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS. Proceedings of the National Academy of Sciences USA (PNAS). 104, 5686-5691.

Schiavon M, Zhang L, Salah E. Abdel-Ghany, Pilon M, Malagoli M, Pilon-Smits EAH (2007): Variation in copper metabolism in Arabidopsis thaliana accessions Columbia, Landsberg erecta and Wassilewskija. Physiologia Plantarum, 129: 342–350.

Puig S, Mira H, Dorcey E, Sanceno´n V, Andre´s-Cola´s N, Garcia-Molina A,. Burkhead J. Gogolin K, Salah E. Abdel-Ghany, Thiele D, Ecker J, Pilon M, Pen˜arrubia L, (2007): Higher plants possess two different types of ATX1-like copper chaperones. Biochemical and Biophysical Research Communications. 354: 385-390.

Narayana M., Ollagnier-de-Choudens S., Sanakis Y., Salah E. Abdel-Ghany, Rousset C., Ye H., Fontecave M , Pilon-Smits EAH., and Pilon M. (2007): Characterization of Arabidopsis thaliana SufE2 and SufE3: functions in chloroplast iron-sulfur cluster assembly and nad synthesis. Journal of Biological Chemistry, Vol. 282, Issue 25, 18254-18264.

Pilon M, Salah E. Abdel-Ghany, Cohu CM, Gogolin KA, Ye H (2006): Copper co-factor delivery in plant cells. Current Opinion in Plant Biology. (3): 256-263.

Ye H, Abdel-Ghany SE, Anderson TD, Pilon-Smits EA, Pilon M (2006): CpSufE activates the cysteine desulfurase CpNIFS for chloroplastic Fe-S cluster formation. Journal of Biological Chemistry. 281(13): 8958-8969.

Lihong Zhang, Abdel-Ghany SE, John L. Freeman, Ashley R. Akley, Michela Shiavon, Elizabeth A.H.Pilon-smits (2006): Investigation of Selenium tolerance mechanism in Arabidopsis thaliana. Physiologia Plantarum 128: 212–223.

Stormy Dawn L., Salah E. Abdel-Ghany, Hanson B., Hwang S., Terry N., and Elizabeth A. H. Pilon-Smits (2006): Constitutive Expression of a High-Affinity Sulfate Transporter in Brassica juncea Affects Metal Tolerance and Accumulation Environmental Quality. 35:726–733.

Salah E. Abdel-Ghany, Muller-Moule P, Niyogi KK, Pilon M, Shikanai T.(2005): Two P-Type ATPases Are Required for Copper Delivery in Arabidopsis thaliana Plant Cell (4): 1233-1251.

Abdel-Ghany SE, Burkhead JL, Gogolin KA, Andres-Colas N, Bodecker JR, Puig S, Penarrubia L, Pilon M. (2005): AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett.579: 2307-2312.

Van Hoewyk D, Garifullina GF, Ackley AR, Abdel-Ghany SE, Marcus MA, Fakra S, Ishiyama K, Inoue E, Pilon M, Takahashi H, Pilon-Smits EA (2005): Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis. Plant Physiology.139:1518-1528.

Abdel-Ghany SE, Hong Ye, Gulnara F. Garifullina, Lihong Zhang, Elizabeth A.H. Pilon-Smits, and Marinus Pilon (2005) Iron-Sulfur Cluster Biogenesis in Chloroplasts. Involvement of the Scaffold Protein CpIscA. Plant Physiology. 138: 161-172.

Abdel-Ghany SE, Day I, Simmons M, Kugrens P, and Reddy ASN (2005): Origin and evolution of kinesin-like calmodulin-binding protein. Plant Physiology. 138, 1711-1722.

Ye H, Garifullina GF, Abdel-Ghany SE, Zhang L, Pilon-Smits EA, Pilon M. (2004): The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin. Planta. 220 (4): 602-608.

Burkhead JL, Abdel-Ghany SE, Morrill JM, Pilon-Smits EA, Pilon M. (2003) The Arabidopsis thaliana CUTA gene encodes an evolutionarily conserved copper binding chloroplast protein. Plant J. 34(6): 856-67.

Van Huysen T, Abdel-Ghany S, Hale KL, LeDuc D, Terry N, Pilon-Smits EA. (2003): Over- expression of cystathionine-gamma-synthase enhances selenium volatilization in Brassica juncea. Planta. 218(1): 71-78.

Pilon-Smits EA, Garifullina GF, Abdel-Ghany SE, Kato S, Mihara H, Hale KL, Burkhead JL, Esaki N, Kurihara T, Pilon M. (2002): Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism. Plant Physiology. 130(3): 1309-1318.

Salah E. Abdel-Ghany, Reddy AS. (2000):   A novel calcium/calmodulin-regulated kinesin-like protein is highly conserved between monocots and dicots. DNA Cell Biology. 19(9):567-578.

Salah E. Abdel-Ghany, Paul Kugrens, Reddy AS. (2000): CpKLP1: Acalmodulin-Binding Kinesin-like Protein from Cyanophora paradoxa (Glaucopyta) Journal of Phycology. 36: 686-692.