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SUMMARY:Expression and Function of cGMP-Dependent Protein Kinase in Molt Re
 gulation in Two Decapod Crustaceans, Gecarcinus Lateralis and Carcinus Mae
 nas
LOCATION:Biology 133
TZID:America/Denver
DTSTART:20250211T110000
UID:2026-05-13-06-53-08@natsci.colostate.edu
DTSTAMP:20260513T065308
Description:You’re Invited to Talia Head’s PhD Exit Seminar\nJoin us fo
 r an engaging presentation by Talia Head\, PhD candidate\, as she discusse
 s her groundbreaking research on the physiological regulation of molting i
 n decapod crustaceans. In her seminar\, Talia will explore the roles of tw
 o key hormones—Molt-Inhibiting Hormone (MIH) and ecdysteroids—and thei
 r influence on the molting process.\n\nTalia’s work delves into the comp
 lex mechanisms behind how these hormones interact\, focusing particularly 
 on the protein PKG and its critical role in regulating ecdysteroid product
 ion. Her findings offer new insights into hormonal regulation in crustacea
 ns and lay the groundwork for future research in animal biology.\nWhy Atte
 nd?\n\n 	Gain a deeper understanding of hormonal control in crustacean bio
 logy.\n 	Learn about the role of PKG in regulating molting and ecdysteroid
  synthesis.\n 	Engage with cutting-edge research that opens doors to new a
 reas of scientific exploration.\n\nCan\\'t attend in-person? Join us on Zo
 om!\n\n 	Zoom link: col.st/Q5C02\n 	Meeting ID: 978 5546 6083\n 	Passcode:
  961339\n\nVisit our website for more information on our seminars and foll
 ow us on social media for more announcements from Biology.\n\n 	Instagram:
  @csubio\n 	Twitter/X: @csubiology\n 	Facebook: Department of Biology at C
 olorado State University\n\nWe hope you can join us to support Talia and l
 earn about this fascinating area of research.\nAbstract\nPhysiological reg
 ulation of molting in decapods is predominantly coordinated by two hormone
 s\, the peptide molt-inhibiting hormone (MIH)\, and steroid molting hormon
 es termed ecdysteroids. MIH is produced and secreted by the X-organ/sinus 
 gland complex located in the eyestalk ganglia and negatively regulates the
  production of ecdysteroids in the molting gland (Y-organ\, YO). MIH signa
 ling begins with a cAMP-dependent triggering phase followed by a cGMP-depe
 ndent summation phase which ultimately leads to inhibition of mTORC1. The 
 involvement of cGMP in MIH signaling implicates the activity of cGMP-depen
 dent protein kinase (PKG)\, although the downstream effects of PKG remain 
 unknown. The goal of this work was to phylogenetically characterize PKG in
  crustaceans\, characterize the physiological effects of PKG-inhibition on
  YO ecdysteroid synthesis\, and identify potential substrates and downstre
 am effects of MIH-dependent PKG activity in the YO. Two genes encoding PKG
 \, pkg1 and pkg2\, were identified in crustaceans and are conserved across
  metazoans. Alternative splicing of the PKG1 N-terminal yields three PKG1Î
 ± and one PKG1β isoform in crustaceans\, consistent with isoforms seen in
  characterized model species. PKG1 sequences with a 14- to 17- amino acid 
 insertion within the kinase domain were identified in ten decapods and one
  stomatopod\, and may indicate that alternative splicing occurs outside of
  the N-terminal. In vitro assays of paired YOs incubated with MIH and PKG 
 inhibitors were used to assess the effects of PKG activity on ecdysteroido
 genesis. In the presence of MIH\, inhibition of both PKG1 and PKG2 increas
 ed ecdysteroid synthesis relative to MIH alone\, whereas PKG2 inhibition e
 nhanced the effects of MIH. These data indicate that the two PKG isoforms 
 have opposing roles in modulating ecdysteroidogenesis via MIH signaling in
  YOs. Specifically\, PKG1 plays a dominant role in MIH signaling by inhibi
 ting ecdysteroid synthesis\, while PKG2 counters that inhibition and maint
 ains basal ecdysteroidogenesis in the intermolt YO. LC-MS/MS analysis of p
 hosphopeptides enriched from PKG-inhibited YOs revealed several novel pote
 ntial substrates of PKG in the YO\, providing ample ground for future work
 .\n\n11:00 am
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