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SUMMARY:Adaptation to Stress in Plant-Associated and Free-Living Microbiome
 s
LOCATION:Biology 136
TZID:America/Denver
DTSTART:20240326T160000
UID:2026-05-13-06-48-56@natsci.colostate.edu
DTSTAMP:20260513T064856
Description:Within their shared environment\, whether wild or agricultural\
 , plants and their local soil-dwelling microbes often encounter the same a
 biotic stresses. This creates the a priori expectation that both plants an
 d their associated microbiomes must adapt simultaneously to these stresses
 . However\, we lack clear examples of how adaptation to a shared environme
 ntal stressor affects interactions between plants and microbial communitie
 s. We use drought as a model stressor to investigate these dynamics at the
  evolutionary\, ecological\, physiological\, and molecular levels.\n\nWe c
 ollected wild soils from across a steep precipitation gradient and allowed
  them to adapt for five months to either intensified or relaxed drought co
 nditions\, with or without a host plant present\, in a factorial design. S
 hotgun metagenome and metatranscriptome sequencing of these soils before a
 nd after the experi-mental evolution were used to identify microbial mecha
 nisms of rapid adaptation to drought in host-associated and free-living co
 ntexts. Finally\, the experimentally-evolved communities were used to inoc
 ulate a new generation of host plants\, which acted as “phytometers” t
 o measure the functional effects of microbiome adaptation on key plant phy
 siological traits such as elemental composition and root anatomy. 4:00 pm
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