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SUMMARY:Modulation of phosphate transport via microRNA- and ubiquitin-media
 ted regulation
LOCATION:Anatomy/Zoology Building W118
TZID:America/Denver
DTSTART:20142101T000000
UID:2026-05-13-07-10-44@natsci.colostate.edu
DTSTAMP:20260513T071044
Description: Phosphorous is an essential plant nutrient that is mainly acq
 uired as a form of inorganic phosphate (Pi) from soil. Recently\, we revea
 led a mechanism by which plants control Pi homeostasis to adapt to externa
 l Pi availability. This mechanism involves interplay between two Pi starva
 tion-induced microRNAs\, miR399 and miR827. In Arabidopsis\, miR399 and mi
 R827 direct the cleavage of PHO2 and NLA transcripts\, respectively. PHO2 
 encodes a ubiquitin E2 conjugase associated with endomembranes while NLA e
 ncodes a RING-type ubiquitin E3 ligase localized in plasma membranes. Loss
 -of-function of PHO2 or NLA results in excess Pi accumulation. We found th
 at PHO2 modulates the activity of xylem loading of Pi via mediating the pr
 otein degradation of PHO1. Furthermore\, both PHO2 and NLA regulate the de
 gradation of PHT1 Pi transporters. Analyses of nla pho2 mutants suggest th
 at NLA and PHO2 function independently but cooperatively to control Pi acq
 uisition. PHO2 regulates the abundance of PHT1s in the secretory pathway d
 estined for the plasma membranes whereas NLA controls the abundance of PHT
 1s in the plasma membranes by endocytosis. Upon Pi deprivation\, upregulat
 ion of miR399 and miR827 suppresses the expression of PHO2 and NLA\, respe
 ctively\, thereby alleviating the negative regulation of PHO1 and PHT1s an
 d reinforcing Pi translocation and uptake activities. Intriguingly\, miR39
 9 can move systemically from shoots to roots\, serving as a signal to bala
 nce the Pi demand in shoots and Pi supply in roots. This study demonstrate
 s the regulation of Pi transport through integration of miRNA-mediated pos
 ttranscriptional and ubiquitin-mediated posttranslational pathways. 4:00 p
 m
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