BEGIN:VCALENDAR VERSION:2.0 PRODID:-//ZContent.net//ZapCalLib 1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT SUMMARY:Genomes and gene expression in marine microbial communities LOCATION:Anatomy/Zoology Building W118 TZID:America/Denver DTSTART:20140201T000000 UID:2026-04-17-22-44-31@natsci.colostate.edu DTSTAMP:20260417T224431 Description:Since 2003\, the J. Craig Venter Institute has conducted a stud y of marine microbial communities using shotgun sequencing.  Samples have been collect from every ocean\, several major inland seas\, lakes\, and t he Amazon River. I will present two studies emerging from this dataset.   The first examines the results of a collaborative survey with the Scripps Institution of Oceangraphy in the eastern subtropical Pacific.  Here\, me tagenomics and metatranscriptomics were used to examine marine microbes ac ross gradients in light and primary productivity.  The results highlight a discordance between genomic abundance and transcriptional activity\, and suggests that relatively rare\, but fast growing bacteria contribute disp roportionately to biogeochemical processes.  The results also suggest tha t a previously overlooked  class of algae\, the Pelagophyceae\, contribut e disproportionately to nitrate reduction.  Second\, I will present  a   metagenomic assembly generated from 227 surface ocean metagenome.  This assembly\, 3Gbp in total including over 9000 contigs greater than 30kbp in length\, provides core and pan genomes for roughly 85% of the taxa found in the ocean\, the vast majority of which are still uncultivated.  Multiv ariate analyses identify the environmental variables\, specifically temper ature and chlorophyll a\, that drive differentiation within these uncultiv ated lineages and between communities.  Finally\, select metabolic insigh ts into what was previously biological dark matter will be presented. 4:0 0 pm END:VEVENT END:VCALENDAR