Office:
Phone: (970) 491-4991
Education
- Ph.D., Utrecht University, The Netherlands
About
In the Pilon-Smits lab we do multidisciplinary research that ranges from cells to whole plants to ecosystems. An important common denominator is selenium (Se): an essential element with anticarcinogenic and antiviral properties, but also a toxin and environmental pollutant. We study plant Se tolerance and accumulation using genetic/genomic tools and biotechnology and biochemistry. At the same time, we are very interested in ecological and evolutionary aspects of Se hyperaccumulation: the capacity of certain species to accumulate Se up to 1% of dry weight. Our findings indicate that Se hyperaccumulation has evolved as a protection from biotic stresses. A better understanding of plant Se metabolism and hyperaccumulation may ultimately lead to the development of pathogen- and pest-resistant, anticarcinogenic, Se-fortified crops, and plants with superior properties for the phytoremediation of Se-polluted soils and waters. Plant species we use in our research are the hyperaccumulator Stanleya pinnata, non-hyperaccumulator relatives Stanleya elata, Brassica juncea and Arabidopsis thaliana, and, recently, Cannabis sativa (hemp).
Publications
- Effects of Selenium Hyperaccumulators on Soil Se Distribution and Vegetation Properties. American Journal of Botany.
- Distribution and chemical form of selenium in Neptunia amplexicaulis from Central Queensland, Australia. Metallomics.
- Getting to the root of selenium hyperaccumulation – Localization and speciation of root selenium and its effects on nematodes Soil Systems, 47, 2019.
- On the Ecology of Selenium Accumulation in Plants PLANTS-BASEL, 7, 2019.
- Selenium Biofortification Differentially Affects Sulfur Metabolism and Accumulation of Phytochemicals in Two Rocket Species (Eruca Sativa Mill. and Diplotaxis Tenuifolia) Grown in Hydroponics PLANTS-BASEL, 3, 2019.
- Understanding selenium metabolism in plants and its role as a beneficial element CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY, 21, 2019.
- Identification and physiological comparison of plant species that show positive or negative co-occurrence with selenium hyperaccumulators. Metallomics, 2019.
- Exploring the Potential of Hemp (Cannabis sativa L.) for Selenium Biofortification and Phytoremediation. Environmental Science and Technology.
- Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator. Science of the Total Environment.
- Selenium Accumulation, Speciation and Localization in Brazil Nuts (Bertholletia excelsa). Plants, 8, 2019.
- Getting to the Root of Selenium Hyperaccumulation – Localization and Speciation of Root Selenium and its Effects on Nematodes Soil Systems, 2019.
- Misunderstood Patagonia film 15 min online, 2019.
- Comparison of ATP sulfurylase 2 from selenium hyperaccumulator Stanleya pinnata and non-accumulator Stanleya elata reveals differential intracellular localization and enzyme activity levels BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 11, 2018.
- Fungal Endophyte Alternaria tenuissima Can Affect Growth and Selenium Accumulation in Its Hyperaccumulator Host Astragalus bisulcatus FRONTIERS IN PLANT SCIENCE, 2018.
- Influence of sulfate supply on selenium uptake dynamics and expression of sulfate/selenate transporters in selenium hyperaccumulator and nonhyperaccumulator Brassicaceae NEW PHYTOLOGIST, 1, 2018.
- Mechanisms of selenium hyperaccumulation in plants: A survey of molecular, biochemical and ecological cues BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 11, 2018.
- Plant selenium hyperaccumulation-Ecological effects and potential implications for selenium cycling and community structure BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 11, 2018.
- Transcriptome-wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome PLANT BIOTECHNOLOGY JOURNAL, 9, 2018.
- Selenium species influence Se uptake, translocation and biofortification in common buckwheat and tartary buckwheat Frontiers in Plant Science, 2018.
- Plant Selenium Hyperaccumulation Affects Rhizosphere: Enhanced Species Richness and Altered Species Composition Phytobiomes, 2018.
- Ecology of selenium in plantsSelenium in plants Book, 2017.
- Evolutionary aspects of plant selenium accumulationSelenium in plants Book, 2017.
- Mechanisms of plant selenium hyperaccumulationSelenium in plants Book, 2017.
- Selenium in Plants Springer, 2017.
- Tansley review: The fascinating facets of plant selenium accumulation - biochemistry, physiology, evolution and ecology. The New phytologist, 2017.
- Selenium biofortification and phytoremediation phytotechnologies Journal of Environmental Quality, 2017.
- Selenium Biofortification in Radish Enhances Nutritional Quality via Accumulation of Methyl-Selenocysteine and Promotion of Transcripts and Metabolites Related to Glucosinolates, Phenolics, and Amino Acids. Frontiers in plant science, 2016.
- Superoxide generated from the glutathione-mediated reduction of selenite damages the iron-sulfur cluster of chloroplastic ferredoxin. Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale, 2016.
- Comparative effects of selenate and selenite on selenium accumulation, morphophysiology, and glutathione synthesis in Ulva australis. Environmental science and pollution research international, 15, 2016.
- Symphyotrichum ericoides populations from seleniferous and nonseleniferous soil display striking variation in selenium accumulation New Phytologist, 1, 2015.
- Progress toward clonable inorganic nanoparticles. Nanoscale, 41, 2015.
- Pseudomonas moraviensis subsp. stanleyae, a bacterial endophyte of hyperaccumulator Stanleya pinnata, is capable of efficient selenite reduction to elemental selenium under aerobic conditions. Journal of applied microbiology, 2, 2015.
- Molybdenum accumulation, tolerance and molybdenum-selenium-sulfur interactions in Astragalus selenium hyperaccumulator and nonaccumulator species. Journal of plant physiology, 2015.
- Symphyotrichum ericoides populations from seleniferous and nonseleniferous soil display striking variation in selenium accumulation. The New phytologist, 1, 2015.
- Electrocoagulation of colloidal biogenic selenium. Environmental science and pollution research international, 4, 2015.
- Do selenium hyperaccumulators affect selenium speciation in neighboring plants and soil? An X-Ray Microprobe Analysis. International journal of phytoremediation, 8, 2015.
- Effects of selenium biofortification on crop nutritional quality. Frontiers in plant science, 2015.
- Evolution of selenium hyperaccumulation in Stanleya (Brassicaceae) as inferred from phylogeny, physiology and X-ray microprobe analysis. The New phytologist, 2, 2015.
- Exploring the importance of sulfate transporters and ATP sulphurylases for selenium hyperaccumulation-a comparison of Stanleya pinnata and Brassica juncea (Brassicaceae). Frontiers in plant science, 2015.
- Microbial-enhanced Selenium and Iron Biofortification of Wheat (Triticum aestivum L.)--Applications in Phytoremediation and Biofortification. International journal of phytoremediation, 1-6, 2015.
- Selenium hyperaccumulators harbor a diverse endophytic bacterial community characterized by high selenium resistance and plant growth promoting properties. Frontiers in plant science, 2015.
- Selenium-fortified wheat: potential of microbes for biofortification of selenium and other essential nutrients. International journal of phytoremediation, 8, 2015.
- Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae) American journal of botany, 11, 2014.
- Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae). American journal of botany, 11, 2014.
- Analysis of selenium accumulation, speciation and tolerance of potential selenium hyperaccumulator Symphyotrichum ericoides. Physiologia plantarum, 1, 2014.
- Characterization of selenium and sulfur accumulation across the genus Stanleya (Brassicaceae): A field survey and common-garden experiment. American journal of botany, 5, 2014.
- Evolutionary aspects of elemental hyperaccumulation. Planta, 2, 2014.
- Sulfur-selenium-molybdenum interactions distinguish selenium hyperaccumulator Stanleya pinnata from non-hyperaccumulator Brassica juncea (Brassicaceae). Planta, 2, 2014.
- Inoculation of selenium hyperaccumulator Stanleya pinnata and related non-accumulator Stanleya elata with hyperaccumulator rhizosphere fungi--investigation of effects on Se accumulation and speciation. Physiologia plantarum, 1, 2014.
- Selenium fertilization alters the chemical composition and antioxidant constituents of tomato (Solanum lycopersicon L.). Journal of agricultural and food chemistry, 44, 2013.
- Inoculation of Astragalus racemosus and Astragalus convallarius with selenium-hyperaccumulator rhizosphere fungi affects growth and selenium accumulation. Planta, 3, 2013.
- Selenium hyperaccumulation by Astragalus (Fabaceae) does not inhibit root nodule symbiosis American journal of botany, 12, 2012.
- Selenium hyperaccumulation by Astragalus (Fabaceae) does not inhibit root nodule symbiosis. American journal of botany, 12, 2012.
- Transcriptome profiling of genes differentially modulated by sulfur and chromium identifies potential targets for phytoremediation and reveals a complex S-Cr interplay on sulfate transport regulation in B. juncea. Journal of hazardous materials, 2012.
- Accumulation of selenium in Ulva sp. and effects on morphology, ultrastructure and antioxidant enzymes and metabolites. Aquatic toxicology (Amsterdam, Netherlands), 2012.
- Selenium distribution and speciation in the hyperaccumulator Astragalus bisulcatus and associated ecological partners. Plant physiology, 4, 2012.
- Interactions of selenium hyperaccumulators and nonaccumulators during cocultivation on seleniferous or nonseleniferous soil--the importance of having good neighbors. The New phytologist, 1, 2012.
- Ecological aspects of plant selenium hyperaccumulation. Plant biology (Stuttgart, Germany), 1, 2012.
- Selenium hyperaccumulator plants Stanleya pinnata and Astragalus bisulcatus are colonized by Se-resistant, Se-excluding wasp and beetle seed herbivores. PloS one, 12, 2012.
- Enhanced decomposition of selenium hyperaccumulator litter in a seleniferous habitatÑevidence for specialist decomposers? Plant and soil, 1-2, 2011.
- Effects of selenium accumulation on reproductive functions in Brassica juncea and Stanleya pinnata. Journal of experimental botany, 15, 2011.
- Selenium accumulation in flowers and its effects on pollination. The New phytologist, 3, 2011.
- Selenium hyperaccumulators facilitate selenium-tolerant neighbors via phytoenrichment and reduced herbivory. Current biology : CB, 17, 2011.
- Adenosine 5'-phosphosulfate reductase (APR2) mutation in Arabidopsis implicates glutathione deficiency in selenate toxicity. The Biochemical journal, 2, 2011.
- Characterization of rhizosphere fungi from selenium hyperaccumulator and nonhyperaccumulator plants along the eastern Rocky Mountain Front Range. American journal of botany, 7, 2011.
- Effects of selenium hyperaccumulation on plant-plant interactions: evidence for elemental allelopathy? The New phytologist, 1, 2011.
- Selenium accumulation in plants--phytotechnological applications and ecological implications. International journal of phytoremediation, 2011.
- Selenium accumulation, distribution, and speciation in spineless prickly pear cactus: a drought- and salt-tolerant, selenium-enriched nutraceutical fruit crop for biofortified foods. Plant physiology, 1, 2011.
- MetallophytesÑa view from the rhizosphere Plant and Soil, 1-2, 2010.
- Selenium hyperaccumulation offers protection from cell disruptor herbivores. BMC ecology, 2010.
- Molecular mechanisms of selenium tolerance and hyperaccumulation in Stanleya pinnata. Plant physiology, 4, 2010.
- Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation. Trends in plant science, 8, 2009.
- Physiological functions of beneficial elements. Current opinion in plant biology, 3, 2009.
- Selenium protects the hyperaccumulator Stanleya pinnata against black-tailed prairie dog herbivory in native seleniferous habitats. American journal of botany, 6, 2009.
- Phytoremediation of selenium using transgenic plants. Current opinion in biotechnology, 2, 2009.
- Effects of agronomic practices on phytoremediation of an aged PAH-contaminated soil Journal of environmental quality, 4, 2008.
- Heavy metal tolerance and accumulation in Indian mustard (Brassica juncea L.) expressing bacterial gamma-glutamylcysteine synthetase or glutathione synthetase. International journal of phytoremediation, 5, 2008.
- Interactions between chromium and sulfur metabolism in Brassica juncea. Journal of environmental quality, 4, 2008.
- Cooperative ethylene and jasmonic acid signaling regulates selenite resistance in Arabidopsis. Plant physiology, 3, 2008.
- The role of selenium in protecting plants against prairie dog herbivory: implications for the evolution of selenium hyperaccumulation. Oecologia, 2, 2008.
- Transcriptome analyses give insights into selenium-stress responses and selenium tolerance mechanisms in Arabidopsis. Physiologia plantarum, 2, 2008.
- Selenium hyperaccumulation reduces plant arthropod loads in the field. The New phytologist, 3, 2008.
- Characterization of metal tolerance and accumulation in Grevillea exul var exul. International journal of phytoremediation, 5, 2007.
- Comparison of plant families in a greenhouse phytoremediation study on an aged polycyclic aromatic hydrocarbon-contaminated soil. Journal of environmental quality, 5, 2007.
- Selenium: deterrence, toxicity, and adaptation. Integrated environmental assessment and management, 3, 2007.
- Characterization of Arabidopsis thaliana SufE2 and SufE3: functions in chloroplast iron-sulfur cluster assembly and Nad synthesis. The Journal of biological chemistry, 25, 2007.
- Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS. Proceedings of the National Academy of Sciences of the United States of America, 13, 2007.
- Variation in selenium tolerance and accumulation among 19 Arabidopsis thaliana accessions. Journal of plant physiology, 3, 2007.
- Transgenic Indian mustard overexpressing selenocysteine lyase or selenocysteine methyltransferase exhibit enhanced potential for selenium phytoremediation under field conditions. Environmental science & technology, 2, 2007.
- Seasonal fluctuations of selenium and sulfur accumulation in selenium hyperaccumulators and related nonaccumulators. The New phytologist, 3, 2007.
- Selenium accumulation protects plants from herbivory by Orthoptera via toxicity and deterrence. The New phytologist, 3, 2007.
- Mapping quantitative trait loci associated with selenate tolerance in Arabidopsis thaliana New Phytologist, 1, 2006.
- Selenium-tolerant diamondback moth disarms hyperaccumulator plant defense. Current biology : CB, 22, 2006.
- Spatial imaging, speciation, and quantification of selenium in the hyperaccumulator plants Astragalus bisulcatus and Stanleya pinnata. Plant physiology, 1, 2006.
- Constitutive expression of a high-affinity sulfate transporter in Indian mustard affects metal tolerance and accumulation. Journal of environmental quality, 3, 2006.
- CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation. The Journal of biological chemistry, 13, 2006.
- Biogenesis of iron-sulfur cluster proteins in plastids. Genetic engineering, 2006.
- CpNifS-dependent iron-sulfur cluster biogenesis in chloroplasts. The New phytologist, 2, 2006.
- Mapping quantitative trait loci associated with selenate tolerance in Arabidopsis thaliana. The New phytologist, 1, 2006.
- Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis. Plant physiology, 3, 2005.
- Iron-sulfur cluster biogenesis in chloroplasts. Involvement of the scaffold protein CpIscA. Plant physiology, 1, 2005.
- Field trial of transgenic Indian mustard plants shows enhanced phytoremediation of selenium-contaminated sediment. Environmental science & technology, 6, 2005.
- The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin. Planta, 4, 2005.
- Exploring the selenium phytoremediation potential of transgenic Indian mustard overexpressing ATP sulfurylase or cystathionine-gamma-synthase. International journal of phytoremediation, 2, 2004.
- Overexpression of ATP sulfurylase in Indian mustard: effects on tolerance and accumulation of twelve metals. Journal of environmental quality, 1, 2004.
- Ecology of rhizosphere bioremediation Phytoremediation: transformation and control of contaminants, 2003.
- Selenium accumulation protects Brassica juncea from invertebrate herbivory and fungal infection New Phytologist, 2, 2003.
- Overexpression of cystathionine-gamma-synthase enhances selenium volatilization in Brassica juncea. Planta, 1, 2003.
- The Arabidopsis thaliana CUTA gene encodes an evolutionarily conserved copper binding chloroplast protein. The Plant journal : for cell and molecular biology, 6, 2003.
- Analysis of transgenic Indian mustard plants for phytoremediation of metal-contaminated mine tailings. Journal of environmental quality, 2, 2003.
- Enhanced selenium tolerance and accumulation in transgenic Arabidopsis expressing a mouse selenocysteine lyase. Plant physiology, 3, 2003.
- Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism. Plant physiology, 3, 2002.
- Stimulation of microbial polycyclic aromatic hydrocarbon biodegradation by plant chemicals.ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY2001.
- Molybdenum sequestration in Brassica species. A role for anthocyanins? Plant physiology, 4, 2001.
- Breeding mercury-breathing plants for environmental cleanup. Trends in plant science, 6, 2000.
- Cadmium tolerance and accumulation in Indian mustard is enhanced by overexpressing gamma-glutamylcysteine synthetase. Plant physiology, 4, 1999.
- Overexpression of ATP sulfurylase in indian mustard leads to increased selenate uptake, reduction, and tolerance Plant physiology, 1, 1999.
- Overexpression of glutathione synthetase in indian mustard enhances cadmium accumulation and tolerance Plant physiology, 1, 1999.
- Rate-limiting steps in selenium assimilation and volatilization by indian mustard Plant physiology, 4, 1998.