Tai Montgomery

Tai MontgomeryAssistant Professor
Office: Biology 240
Phone: (970) 491-7198
Website: http://montgomerylab.org
Email: tai.montgomery@colostate.edu

RESEARCH SUMMARY Small_RNA_Gene_Silencing-new RNA interference (RNAi) and related pathways engage small RNAs to control endogenous genes and distinguish invading genes from “self” to act as an immune system for viruses and tranpsosons. Small RNAs, including microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), and small-interfering RNAs (siRNAs), affect gene expression transcriptionally through epigenetic modifications and post-transcriptionally through translational repression and mRNA decay. Small RNAs are so adaptable that together with their protein cofactors they have proliferated and diversified extensively so as to control a wide range of biological processes. The mechanisms in which small RNAs are made and how they function is conserved between animals as distantly related as Caenorhabditis elegans and humans. Our research focuses on understanding these important regulatory pathways and how they intersect processes as diverse as heterochromatin formation, transcriptional elongation, translation of mRNAs, and sorting of RNAs within a cell. The small RNA regulation of these processes is now emerging to be important in various disease states as well. Although considerable progress has been made in our understanding of small RNA pathways in the past 15 years, there are still many exciting discoveries to be made. Check us out on Facebook - Montgomery Lab  fb-art

Selected Publications

Brown K.C. and Montgomery T.A. (2018). The long and short of lifespan regulation by Argonautes. PLoS Genetics, 14, e1007415.

Svendsen, J.M. and Montgomery, T.A. (2018). piRNA rules of engagement. Developmental Cell, 44, 657-658.

Brown, K.C, Svendsen, J.M, Tucci, R.M, Montgomery, B.E., and Montgomery, T.A. (2017). ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline. Nucleic Acids Research, 45, 9093-9107.

Weiser, N.E., Yang, D.X., Feng, S., Kalinava, N., Brown, K.C., Khanikar, J., Freeberg, M.A., Snyder, M.J., Csankovszki, G., Chan, R.C., Gu, S.G., Montgomery, T.A., Jacobsen, S.E., and Kim, J.K. (2017). MORC-1 integrates nuclear RNAi and transgenerational chromatin architecture to promote germline immortality. Developmental Cell 41, 408-423.

Brown K.C. and Montgomery T.A. (2017). Transgenerational inheritance: perpetuating RNAi. Current Biology 27, R383-R385.

Phillips, C.M., Brown, K.C., Montgomery, B.E., Ruvkun, G., and Montgomery, T.A. (2015). piRNAs and piRNA-dependent siRNAs protect conserved and essential C. elegans genes from misrouting into the RNAi pathway. Developmental Cell 34, 457-465.

Phillips, C.M., Montgomery, B.E., Breen, P.C., Roovers, E.F., Rim, Y.S., Ohsumi, T.K., Newman, M.A., van Wolfswinkel, J.C., Ketting, R.F., Ruvkun, G., and Montgomery, T.A. (2014). MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation. Current Biology 24, 839-844.

Montgomery, T.A. and Ruvkun, G. (2013). MicroRNAs visit the ER. Cell 153, 511-512.

Shi, Z., Montgomery, T.A., Qi, Y., and Ruvkun, G. (2013). High-throughput sequencing reveals extraordinary fluidity of miRNA, piRNA and siRNA pathways in nematodes. Genome Research 23, 497-508.

Carbonell, A., Fahlgren, N., Garcia-Ruiz, H., Gilbert, K.B., Montgomery, T.A., Nguyen, T., Cuperus, J.T., and Carrington, J.C. (2012).  Functional analysis of three Arabidopsis ARGONAUTES using slicer-defective mutants. Plant Cell 24, 3613-3629.

Phillips, C.M., Montgomery, T.A., Breen, P.C., and Ruvkun, G. (2012). MUT-16 promotes formation of perinuclear Mutator foci required for RNA silencing in the C. elegans germline. Genes and Development 26,1433-1444.

Montgomery, T.A., Rim, Y.S., Zhang, C., Dowen, R.H., Phillips, C.M., Fischer, S.E., and Ruvkun, G. (2012). PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1. PLoS Genetics 8, e1002616.

Zhang, C., Montgomery, T.A., Fischer, S.E., Garcia, M.D.A., Riedel, C.G., Fahlgren, N., Sullivan, C.M., Carrington, J.C., and Ruvkun, G. (2012). The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by promoting secondary siRNA amplification. Current Biology 22, 881-890.

Fischer, S.E., Montgomery, T.A., Zhang, C., Fahlgren, N., Breen, P.C., Hwang, A., Sullivan, C.M., Carrington, J.C., and Ruvkun, G. (2011). The ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications. PLoS Genetics 7, e1002369.

Zhang, C.*, Montgomery, T.A.*, Gabel, H.W.*, Fischer, S.E., Phillips, C.M., Fahlgren, N., Sullivan, C.M., Carrington, J.C., and Ruvkun, G. (2011). mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans. Proceedings of the National Academy of Sciences 108, 1201-1208.

Cuperus, J.T.*, Carbonell, A.*, Fahlgren, N., Garcia-Ruiz, H., Burke, R.T., Takeda, A., Sullivan, C.M., Gilbert, S.D., Montgomery, T.A., and Carrington, J.C. (2010). Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. Nature Structural and Molecular Biology 17, 997-1003. 

Cuperus, J.T., Montgomery, T.A., Fahlgren, N., Burke, R.T., Townsend, T., Sullivan, C.M., and Carrington, J.C. (2010). Identification of MIR390a precursor processing-defective mutants in Arabidopsis by direct genome sequencing. Proceedings of the National Academy of Sciences 107, 466-471.

Chitwood, D.H., Nogueira, F.T., Howell, M.D., Montgomery, T.A., Carrington, J.C., and Timmermans, M.C. (2009). Pattern formation via small RNA mobility. Genes and Development 23, 549-554.

Fahlgren, N., Sullivan, C.M., Kasschau, K.D., Chapman, E.J., Cumbie, J.S., Montgomery, T.A., Gilbert, S.D., Dasenko, M., Backman, T.W., Givan, S.A., and Carrington, J.C. (2009). Computational and analytical framework for small RNA profiling by high-throughput sequencing. RNA 15, 992-1002.

Montgomery, T.A., Yoo, S.J., Fahlgren, N., Gilbert, S.D., Howell, M.D., Sullivan, C.M., Alexander, A., Nguyen, G., Allen, E., Ahn, J.H., and Carrington, J.C.  (2008). AGO1-miR173 complex initiates phased siRNA formation in plants. Proceedings of the National Academy of Sciences 105, 20055-20062.

Montgomery, T.A. and Carrington, J.C. (2008). Splicing and dicing with a SERRATEd edge. Proceedings of the National Academy of Sciences 105, 8489-8490.

Montgomery, T.A., Howell, M.D., Cuperus, J.T., Li, D., Hansen, J.E., Alexander, A.L., Chapman, E.J., Fahlgren, N., Allen, E., and Carrington, J.C. (2008). Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation. Cell 133, 128-141.

Liu, P.P., Montgomery, T.A., Fahlgren, N., Kasschau, K.D., Nonogaki, H., and Carrington, J.C. (2007). Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages. Plant Journal 52, 133-146.

Fahlgren, N.*, Montgomery, T.A.*, Howell, M.D.*, Allen, E., Dvorak, S.K., Alexander, A.L., and Carrington, J.C. (2006). Regulation of AUXIN RESPONSE FACTOR3 by TAS3 ta-siRNA affects developmental timing and patterning in Arabidopsis. Current Biology 16, 939-944.