Professor and Director, Life Science Core; CNS Professor LaureateOffice: Biology Building 230Phone: 970-491-2513Education: Ph.D., Cornell UniversityEmail: Deborah.Garrity@ColoState.EDU
The development of the embryonic heart involves both genetic and biomechanical factors. To study how rhythm and function develops in the embryonic heart, we use the zebrafish model to track changes as the heart forms, under normal or abnormal conditions. Our group has an ongoing interest in the contributions of T-box transcription factors, calcium channel subunits, and the sodium-calcium exchanger (NCX1) for establishing normal rhythm and contractility in the heart. Recently, we have become interested in how biomechanical forces (e.g. blood flow and contractility), which are not directly encoded by gene products, nevertheless contribute to normal development of this organ. We have developed imaging-based tools to capture and quantitatively analyze physiological parameters of heart function. Combining these tools with forward and reverse genetics, we hope to understand the timing, scope and regions of the heart most impacted by biomechanical forces.
Johnson, D. Bark, D. Garrity and L. Dasi, “Altered Mechanical state in the embryonic heart results in time-dependent decreases in cardiac function”, Biomechanics and Modeling in Mechanobiology, May (2015).
Johnson, D. Garrity, and L.P. Dasi, “Timing of Mechanical Cues Is An Epigenetic Factor Regulating Cardiac Morphogenesis and Function,” Proceedings of the World Congress of Biomechanics, Boston MA, July (2014).
Bryce W. Schroder, Brennan M. Johnson, Deborah M. Garrity, Lakshmi P. Dasi, & Diego Krapf. “Force Spectroscopy in the Bloodstream of Live Zebrafish with Optical Tweezers”, Frontiers in Optics Conference, Tucson AZ, Oct (2014).
Parrie L, Renfrew E, Vander Wal A, Mueller R, Garrity, D. Zebrafish tbx5 paralogs demonstrate independent essential requirements in cardiac and pectoral fin development. Developmental Dynamics, 242:5, 485-502 (2013).
Johnson B, Garrity D, Dasi P. The transitional cardiac pumping mechanics in the embryonic heart. Cardiovascular Engineering and Technology, 4 (3), 246-255. (2013)
Chernyavskaya Y, Ebert EM, Milligan E, Garrity DM. The Voltage-Gated Calcium Channel CACNB2 (β2.1)protein is Required in the Heart for Control of Cell Proliferation and Heart Tube Integrity. Developmental Dynamics 241(4): 648-662. (2012).
Johnson B, Garrity D, Dasi P. Quantifying function in the embryonic heart. Journal of Biomechanical Engineering, 135(4), 041006-11. (2013)
Rothschild SC, Easley CA, Francescatto L, Lister JA, Garrity DM, Tombes RM. Tbx5-mediated expression of Ca2+/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin development, Developmental Biology, 330(1): 175-184 (2009). PMID: 19345202
Ebert AM, McAnelly CA, Handschy A, Mueller RL, Horne WA and Garrity DM. Genomic organization, expression and phylogenetic analysis of Ca2+ channel β4 (CACNB4) genes in thirteen vertebrate species. Physiological Genomics 2008 Aug 5.
Ebert AM, McAnelly CA, Srinivasan A, Mueller RL, Garrity DB and Garrity DM. The calcium channel β2 (CACNB2) subunit repertoire in teleosts. BMC Molecular Biology, 9:38 (2008).
Qu X, Jia H, Garrity DM, Tompkins K, Batts L, Appel B, Zhong T, Baldwin SH. ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish. 15;317(2):486-96(2008).
Ebert AM, McAnelly CA, Srinivasan A, Linker JL, Horne WA and Garrity DM. Ca2+ channel-independent requirement for MAGUK-family CACNB4 genes in initiation of zebrafish epiboly. Proc. Natl. Acad. Sci. U.S.A. 105 (1), 198-203 (2008)
Ebert AM, Hume GL, Warren KS, Cook NP, Burns CG, Mohideen MA, Siegal G, Yelon D, Fishman MC, Garrity DM. Calcium extrusion is critical for cardiac morphogenesis and rhythmicity in embryonic zebrafish hearts. PNAS 102:17705-17710 (2005).
Garrity DM, Childs S, and Fishman MC. 2002. Heartstrings mutation in zebrafish causes heart/fin Tbx5 deficiency syndrome. Development 129:4635-4645.
Childs S, Chen JN, Garrity DM, and Fishman MC. 2002. Patterning of angiogenesis in the zebrafish embryo. Development 129:973-982.
Roman BL, Pham VN, Lawson ND , Kulik M, Childs S, Lekven AC, Garrity DM, Moon RT, Fishman MC, Lechleider RJ, and Weinstein BM. 2002. Disruption of acvrl1 increases endothelial cell number in zebrafish cranial vessels. Development 129:3009-3019.
Chen JN, van Bebber F, Goldstein AM, Serluca FC, Jackson D, Childs S, Serbedzija G, Warren KS, Mably JD, Garrity DM, Lindahl P, Mayer A, Haffter P, and Fishman MC. 2001. Genetic steps to organ laterality in zebrafish. Comp Funct Genom 2: 60-68.